Insertion junction: CLIP2.066054_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.066054
Chromosome:chromosome 16
Location:139590
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g695218 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED outside_mRNA
Cre16.g695250 (1 of 726) IPR011009 - Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAGGGCACACGCGCATTACGGACTCTTGGACGCCGTGATCAACTCAT
Internal bar code:TCGGGTGTATGGGTGGTCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:115
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGACACACTGAGGCTCAAGT
Suggested primer 2:TGTCGCCACATCGATACGAG