| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066086 |
| Chromosome: | chromosome 12 |
| Location: | 3394663 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497900 | (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold | 3'UTR | |
| Cre12.g497950 | (1 of 1) PTHR10706 - F-BOX FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTCTTGAAGTTCAGTCAAGTTGCGCTGTCCCGAGGTTGCAGCAGCA |
| Internal bar code: | TCCTACCAGGAAACCCGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1667 |
| LEAP-Seq percent confirming: | 96.6102 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCCATACGCCTCGTAC |
| Suggested primer 2: | TAGCTGGCTAGTGGCTACCA |