| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066088 |
| Chromosome: | chromosome 6 |
| Location: | 8757467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310800 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR | |
| Cre06.g310850 | (1 of 12) IPR003439//IPR003593//IPR027417 - ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGTCGAGGTGTACGCGGCGGTGCAGGCTTGCAGCCCGCCAGAAGGCG |
| Internal bar code: | GGGCATAATAGAAACGACATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1437 |
| LEAP-Seq percent confirming: | 95.9184 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGGCGTATGAGTAAGACT |
| Suggested primer 2: | GTCAGACGACGATGAGGACC |