Insertion junction: CLIP2.066088_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066088
Chromosome:chromosome 6
Location:8757490
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g310800 (1 of 781) IPR000104 - Antifreeze protein, type I 5'UTR
Cre06.g310850 (1 of 12) IPR003439//IPR003593//IPR027417 - ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGACTGAATCCACCAATCAAACTCCACCGGCATCGCATCACTATACA
Internal bar code:GGGCATAATAGAAACGACATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1673
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGACGACGATGAGGACC
Suggested primer 2:CGCGGCGTATGAGTAAGACT