| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066089 |
| Chromosome: | chromosome 14 |
| Location: | 2317495 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622800 | (1 of 1) IPR000104//IPR002921//IPR029058 - Antifreeze protein, type I // Fungal lipase-like domain // Alpha/Beta hydrolase fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTACGGAGCCGGGGTGGGACTGGGTGGGCACGACCGCGCGAGGAGGG |
| Internal bar code: | AGTATGCAGGTAGTATTGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3025 |
| LEAP-Seq percent confirming: | 36.5854 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTACCTGTCCACGCGT |
| Suggested primer 2: | TCTTCATTGAGCGCAAGGGT |