| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066091 |
| Chromosome: | chromosome 17 |
| Location: | 4094830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728864 | OPR105 | (1 of 781) IPR000104 - Antifreeze protein, type I; OctotricoPeptide Repeat protein 105 | 3'UTR |
| Cre17.g728900 | FSA1,TAL3 | (1 of 1) PTHR10683//PTHR10683:SF2 - TRANSALDOLASE // SUBFAMILY NOT NAMED; Transaldolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTCCGCATCTGGCCAGGTAAGGCGCACTGGTGGGGGGACGGCCCCC |
| Internal bar code: | GCCCTGTTTTGGGGCACACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2239 |
| LEAP-Seq percent confirming: | 87.7778 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGTTGGTTGAGGTGCTT |
| Suggested primer 2: | GCCTCTGCCTGTGTTTGTTG |