Insertion junction: CLIP2.066097_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066097
Chromosome:chromosome 16
Location:6200554
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g676750 (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTTCCCACGCTCACAACTGCACACCAACTCAACTGCAATGGCAGCTC
Internal bar code:TAACTACTGTAGCGTTTGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1417
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGACGCACAGCTTCAACC
Suggested primer 2:GGCCATGGCTCAGAACTCTT