| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066105 |
| Chromosome: | chromosome 3 |
| Location: | 933721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146627 | (1 of 1) PTHR20852:SF16 - TYPE-1 GLUTAMINE SYNTHETASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAAGCTGCGTGTGAGCTGCGTGTTAAGTAAGATGAGATAGAGACGC |
| Internal bar code: | CTTCGTGTAAGGTTGGCGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2726 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAACAACCGTGTGTCAC |
| Suggested primer 2: | GTGAGAGGTTGGACTGTGGG |