| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066109 |
| Chromosome: | chromosome 5 |
| Location: | 2763173 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240750 | TEF17 | Predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCCCAGGCATAAACTCAGGCAGCGCGCCTGTCCCCACCTGCCACCAC |
| Internal bar code: | GGGTCGGCGGATTGCACCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCATGTGGTGTCATGT |
| Suggested primer 2: | CGGCGCTGTTCTGCTATTTC |