| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066113 |
| Chromosome: | chromosome 8 |
| Location: | 904476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAACACGCTACAGCCCAACCACACGACCATGGCTTCGCTGCTGGCGA |
| Internal bar code: | GGTGGGAGAAAGGTTCTGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3378 |
| LEAP-Seq percent confirming: | 96.5116 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCCTACCACTTGCGTG |
| Suggested primer 2: | TACCGTACATGATGGCGTCG |