Insertion junction: CLIP2.066116_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066116
Chromosome:chromosome 3
Location:6400181
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192750 (1 of 4) 2.7.11.13 - Protein kinase C / PKC 3'UTR
Cre03.g800361 (1 of 1) IPR001584//IPR001878//IPR001986//IPR012337//IPR013103//IPR025724 - Integrase, catalytic core // Zinc finger, CCHC-type // Enolpyruvate transferase domain // Ribonuclease H-like domain // Reverse transcriptase, RNA-dependent DNA polymerase // GAG-pre-integrase domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAAGTTGGTTCGGCAAGCCTGAATTGCTAGCGGGTGCGTTACACACGA
Internal bar code:AATGCACGACTTCCATAATAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:6014
LEAP-Seq percent confirming:0.884956
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:113

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCCTTGTTTGTTTCCCCT
Suggested primer 2:ATCCCCTTACTCCCCCTTCC