| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066124 |
| Chromosome: | chromosome 2 |
| Location: | 5387766 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113652 | (1 of 2) PTHR24320:SF1 - 3-KETODIHYDROSPHINGOSINE REDUCTASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCAATCCAGCTGTAGGGGCATGCCGCACCCCAATGACGCTGTACAGC |
| Internal bar code: | TGGCGTGGAGCCCATTATTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1802 |
| LEAP-Seq percent confirming: | 96.9388 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCGATCGGTGTGTTTTT |
| Suggested primer 2: | CGCTGCATTACAACGATGGG |