| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066131 |
| Chromosome: | chromosome 10 |
| Location: | 5979500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461400 | (1 of 1) PTHR22957:SF212 - [PROTEIN ADP-RIBOSYLARGININE] HYDROLASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCATCTCCATCTCGGAGCTCCTAACAACGCCGCAGCTCGCCGGGCAGG |
| Internal bar code: | ATAAGGGATCGATACGTCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4309 |
| LEAP-Seq percent confirming: | 92.1569 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTCCACCATCTCTGCCA |
| Suggested primer 2: | AGGACGACTTCACATGCGTT |