Insertion junction: CLIP2.066134_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066134
Chromosome:chromosome 9
Location:2183708
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g395450 (1 of 9) PF01344 - Kelch motif (Kelch_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATGCTAGCATGCACACGAGTGCAATCAAACATCATTTCCCAAAACGCC
Internal bar code:CTATGCATCAGCGATTAAAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:5023
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCATGGATTCGGGGGATG
Suggested primer 2:AGGATCCAGGCACTCAGCTA