| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066137 |
| Chromosome: | chromosome 6 |
| Location: | 2347734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267750 | (1 of 1) PF00050//PF00089 - Kazal-type serine protease inhibitor domain (Kazal_1) // Trypsin (Trypsin) | 3'UTR | |
| Cre06.g267800 | MML1,MITC8,MCP8 | (1 of 1) K15119 - solute carrier family 25, member 39/40 (SLC25A39_40); MTM-Like Mn transporter 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTTGCTGGTGCGTCCTCAGGCCTGTAACCAATTGGATCAGTGCCTCC |
| Internal bar code: | TAGTTAAGCTGTGTAGGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2961 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAGCTATGAGGTCCTCA |
| Suggested primer 2: | GGCGATTTTCTTGTCGGACG |