Insertion junction: CLIP2.066143_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066143
Chromosome:chromosome 3
Location:2826146
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g162000 TPT8,TPT7 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 5) PTHR11132//PTHR11132:SF101 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGAGAACCCGCCCGCGGCGCGGGTCAAATGCGTTGCTAGCTTGATAGGT
Internal bar code:TTAATGTTGGTCACTCTGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3536
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGGTTTCTGTCATGTCCG
Suggested primer 2:TGTACAAGTGAGTGCCGTCC