| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066153 |
| Chromosome: | chromosome 1 |
| Location: | 4106906 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGGGCCCATTATGCCCTGTGTAATACACATCTGACGCTGTTTCGAC |
| Internal bar code: | AATCTCGTTCATAGTATGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 36.8421 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCTCCCACACACATCC |
| Suggested primer 2: | ATGGTTCATGGGCTGGATGG |