| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066155 |
| Chromosome: | chromosome 6 |
| Location: | 3077085 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275100 | Nucleolin; (1 of 1) K11294 - nucleolin (NCL, NSR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCGAGCCCATTGGTGTCCCACTGGAACACAGCATCTTAAGGGACAGA |
| Internal bar code: | CGCTATTCCATGCGCGGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCGCTAATGACGGGATGC |
| Suggested primer 2: | ATGTGGTGGTGGGTTGTGTT |