| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066158 |
| Chromosome: | chromosome 1 |
| Location: | 50449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000150 | ZRT2,CrZIP2 | Zinc-nutrition responsive permease transporter; (1 of 6) K14709 - solute carrier family 39 (zinc transporter), member 1/2/3 (SLC39A1_2_3, ZIP1_2_3) | 3'UTR |
| Cre01.g000200 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain; Esterase, SGNH hydrolase-type | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGCAGCATCACCGGCATGCCGTGCTTTGAAGGCCAAAGCCTGTAGCG |
| Internal bar code: | AACAAAGGACGGGCACGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3152 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCCTCACCTGCCTCCA |
| Suggested primer 2: | GTGTTTGACAAGCTGGCAGG |