| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066160 |
| Chromosome: | chromosome 4 |
| Location: | 3352665 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227350 | CCR4 | Putative NOT-complex component; (1 of 1) K12580 - CCR4-NOT transcription complex subunit 3 (CNOT3, NOT3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGAGGTCGGGGCTGGAGAAGGGTGGGGTGTTTCAGCCGCTGGGGCGG |
| Internal bar code: | TTCTTATTCGGAGGCGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCGTCACTGGTCACGG |
| Suggested primer 2: | CACTCACATCACGTCAGGCT |