Insertion junction: CLIP2.066169_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066169
Chromosome:chromosome 5
Location:276459
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g243351 (1 of 3) IPR000477//IPR002156//IPR012337 - Reverse transcriptase domain // Ribonuclease H domain // Ribonuclease H-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAACCGCTGGCGTGTCGACGCCGTGTTGCAGCCAGGCGGCGGTGTTC
Internal bar code:TGGGGCGTGACGAAGTAGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2211
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTCAACCATAGCAACCT
Suggested primer 2:CGCCCAGAGAGTCCATAACC