| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066169 |
| Chromosome: | chromosome 5 |
| Location: | 276474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243351 | (1 of 3) IPR000477//IPR002156//IPR012337 - Reverse transcriptase domain // Ribonuclease H domain // Ribonuclease H-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCAGCGGTTCGCAATGCCGCAGTCTGGCACTTCGATTCCGCACCAGCC |
| Internal bar code: | ACGTTAATTGTCTTGGAAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 13.1579 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCAGAGAGTCCATAACC |
| Suggested primer 2: | GGGCTCAACCATAGCAACCT |