| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066173 |
| Chromosome: | chromosome 8 |
| Location: | 2481700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372300 | (1 of 1) IPR000104//IPR000620 - Antifreeze protein, type I // EamA domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGTTGAGTTGCATCGTCCGGATACGGTGCCAAGCAGCAGGCGACCC |
| Internal bar code: | TGTTCGAGGGTGTGTATTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2450 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGTAGACGGACAATCGC |
| Suggested primer 2: | CGTGGGGTTAATGTGGGTGA |