Insertion junction: CLIP2.066178_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.066178
Chromosome:chromosome 3
Location:5189930
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g181450 TCTN1,POB7 (1 of 1) K19382 - tectonic-1/3 (TCTN1_3); Proteome of basal body 7 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAAATAATTCCTGTAAGCCGTGCTTGTGGTCAATTGCACTGCCGGAC
Internal bar code:GTACTATCTTAATATACGAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:10.6383
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAGGCGAGATTACGATGC
Suggested primer 2:CTGAACAAACAAGCGCGTCA