| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066198 |
| Chromosome: | chromosome 5 |
| Location: | 2457107 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238935 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGACGACGTTTTGATACCAGACAGAGTCCTATCACTCCTATGCGGCT |
| Internal bar code: | TCACGAATTGGACGACATACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1448 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCTTGGGACTGAACGT |
| Suggested primer 2: | GCAGAAGTGTAGCGCGAATG |