| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066204 |
| Chromosome: | chromosome 9 |
| Location: | 2203464 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395300 | (1 of 1) PTHR11736//PTHR11736:SF14 - MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN // MAGE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCAGTGCTCGTATCTTTGTAGATGCCCTCAGGCACCCGGCATCTACAC |
| Internal bar code: | GCGTTTGCGTTTTTTTGGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGATGTTGCTGCTGTGGGG |
| Suggested primer 2: | TGTTCGATCCTGTCCGTGTG |