| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066206 |
| Chromosome: | chromosome 10 |
| Location: | 3928787 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446700 | ANK28 | (1 of 2) PTHR24161:SF17 - PALMITOYLTRANSFERASE; Predicted protein with ankyrin repeats | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACTTGCCGACCTGGCCCTGCGCCAAGACGCGAAAGGCGGGAAAACCC |
| Internal bar code: | TTTAGTCTGGTAAGTATCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3703 |
| LEAP-Seq percent confirming: | 97.0874 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGGTGTGTCAGTGTGTA |
| Suggested primer 2: | AACATCTTCGGTCTGCAGGG |