Insertion junction: CLIP2.066209_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.066209
Chromosome:chromosome 8
Location:874660
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361450 (1 of 1) IPR008867//IPR009836 - Thiazole biosynthesis // Protein of unknown function DUF1399 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCGCCCTATTCCCACATGGTTGCTCTGTTCCAAGAGTTCGGTACTCTC
Internal bar code:TTATCTTGTGACCGAAGGTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1888
LEAP-Seq percent confirming:97.2973
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GGATAGCGTACCCAGCTGAC
Suggested primer 2:CAGTATCATGCGCAACCTGC