| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066210 |
| Chromosome: | chromosome 6 |
| Location: | 4673177 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278600 | (1 of 1) PF11221 - Subunit 21 of Mediator complex (Med21) | 3'UTR | |
| Cre06.g278650 | (1 of 1) PTHR11635//PTHR11635:SF127 - CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN // CAMP-DEPENDENT PROTEIN KINASE TYPE II REGULATORY SUBUNIT | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATACGAGAGCCGCCCCCTGCATGAAATGCATCACGGCAGGGAACATTTT |
| Internal bar code: | ACCGGCGACTTCCGGGCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5505 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCCGGTAGTGTCTTCA |
| Suggested primer 2: | CGCGGGAAACTTTTGCAGAA |