| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066225 |
| Chromosome: | chromosome 4 |
| Location: | 3311618 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGAGACATTAGCCCCCAGGCAGGGTTGCGTAGTTCGTAGTACGCGTA |
| Internal bar code: | GGTGATCATCTAAAGGTGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1438 |
| LEAP-Seq percent confirming: | 81.4815 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGACCCTCTCTCCATAAC |
| Suggested primer 2: | ATGGTTCAGGAAGGAGCGTG |