| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066228 |
| Chromosome: | chromosome 6 |
| Location: | 2110406 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266100 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR | |
| Cre06.g266150 | (1 of 7) 2.7.11.1//2.7.11.17 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCATATTGTAACTGCAATGATTGTGCTTGGCGTGCGTGTGTCAGGGT |
| Internal bar code: | GTCATCGCCCAGTAGGCTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4520 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGGCGTACTCAGTGTT |
| Suggested primer 2: | ACTCGGTGTTTGAGGACGAC |