| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066229 |
| Chromosome: | chromosome 10 |
| Location: | 3874936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446250 | (1 of 1) K13339 - peroxin-6 (PEX6, PXAAA1) | 3'UTR | |
| Cre10.g446275 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTCGTGACCTAACAGAGGCCTTATGAGCTACACAAAGGGCTGGCTG |
| Internal bar code: | CTACAGCCTGCCCACTACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2051 |
| LEAP-Seq percent confirming: | 89.1892 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAAACCAGGCCAAGCAG |
| Suggested primer 2: | CTCGTGTGTGGAGGTCTGAC |