| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066229 |
| Chromosome: | chromosome 10 |
| Location: | 3874942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446250 | (1 of 1) K13339 - peroxin-6 (PEX6, PXAAA1) | 3'UTR | |
| Cre10.g446275 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATCTATAAAGTGTGGCATACGGTGCATGACAATACTGGCACAAACGA |
| Internal bar code: | CTACAGCCTGCCCACTACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1538 |
| LEAP-Seq percent confirming: | 92.9825 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGTGTGGAGGTCTGAC |
| Suggested primer 2: | GTTGAAACCAGGCCAAGCAG |