| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066238 |
| Chromosome: | chromosome 16 |
| Location: | 3972219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689700 | HPR5 | (1 of 1) 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP(+)); Hydroxypyruvate reductase 5 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCGGGTCCGCAGCATCTCCACAATGGTTGGCTCCCGACCCCCGACA |
| Internal bar code: | AGTGACCCGAAACGGACTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5549 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTTTATCCGCTTGC |
| Suggested primer 2: | AGCAAGTTCACTGAGGACCG |