Insertion junction: CLIP2.066242_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066242
Chromosome:chromosome 1
Location:8064451
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g062172 HBV1 (1 of 27) K11252 - histone H2B (H2B); Histone H2B variant 3'UTR
Cre01.g062537 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCATGTGAAGTCATGTCATAGGCGTGGATAGTGAGGGCCAGAGCCCA
Internal bar code:GGGGCGCTAGATTCTATCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2925
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGACAAGATGGCCAACGA
Suggested primer 2:ACCTTCTACCCCTCCATCCC