| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066249 |
| Chromosome: | chromosome 13 |
| Location: | 1948283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576200 | (1 of 3) PF01048 - Phosphorylase superfamily (PNP_UDP_1) | 3'UTR | |
| Cre13.g576250 | (1 of 1) K15202 - general transcription factor 3C polypeptide 5 (transcription factor C subunit 1) (GTF3C5, TFC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCCCCAATCGCTGAGGAAACGCAGCTCGCTCCTGACGGTGTGACATG |
| Internal bar code: | GATTAAGCGGGACGCCCCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2834 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGTTCACGGTGGATTA |
| Suggested primer 2: | ACGACGACGATGACGATGAG |