| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066250 |
| Chromosome: | chromosome 12 |
| Location: | 8351260 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550750 | SDHAF2,EMI5 | (1 of 2) PF03937 - Flavinator of succinate dehydrogenase (Sdh5); Succinate dehydrogenase assembly factor 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGACTTGCACCCCTGTACGCCCCTGCTTTCGGGCTCAACACTGCCCT |
| Internal bar code: | GAGTGCAGTGCTGTGCGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2900 |
| LEAP-Seq percent confirming: | 0.892857 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGCTTAGGTGCTGTAA |
| Suggested primer 2: | GCGGTCATGGCGTACTTTTC |