| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066254 |
| Chromosome: | chromosome 2 |
| Location: | 7384723 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391949 | (1 of 1) PTHR23139//PTHR23139:SF61 - RNA-BINDING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCCCCGCCCCTCCCCGCCTCCCCCTTGCAGGTGATTATGGAGTTCAA |
| Internal bar code: | TATACCTTCCCGGGTTACTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1294 |
| LEAP-Seq percent confirming: | 29.1667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATGTGCACTCTTGGAT |
| Suggested primer 2: | CGGTAGCTGATGGAGAGCTG |