| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066257 |
| Chromosome: | chromosome 16 |
| Location: | 7360900 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679557 | FAS5 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTCGTATGGTGCCGAGTGCGATCCCTGCCACTATTACCTTTTGACAT |
| Internal bar code: | GGGGCGGTACCCATCGGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1662 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAATGCCCGCGAAAAGAC |
| Suggested primer 2: | CTGTAGGCTAGCCATGACGG |