| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.066267 |
| Chromosome: | chromosome 5 |
| Location: | 3401317 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232950 | (1 of 1) K13110 - microfibrillar-associated protein 1 (MFAP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGCGGCGGCGGGCGAGGAGGTGGGGGAGGACGTGGAGGAGGAGGAG |
| Internal bar code: | TTTATGATACGATCGCCCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1442 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACTGTGGTGAAGGCGA |
| Suggested primer 2: | GCACAACCCAACCTCACAAC |