| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066284 |
| Chromosome: | chromosome 9 |
| Location: | 472557 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749197 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGAGGCGGAGGCGAGGAGATGGGGAAGCATGGGTATGAAAAGGCTGA |
| Internal bar code: | AGTAGCGGTAACCCACCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATCAACACCCACATAC |
| Suggested primer 2: | AGGAGGAAGGCATCAGGGAT |