| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066288 |
| Chromosome: | chromosome 16 |
| Location: | 6101942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677500 | AKN,APK1,EZY13,AKN2 | (1 of 1) 2.7.1.25 - Adenylyl-sulfate kinase / APS kinase; Adenosine 5'-phosphosulfate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTCAATATCAAGCTCGCGAAAGAGCAAAGAGTATACAGGTCACTAT |
| Internal bar code: | GAAGATTTTGTGGATACTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5593 |
| LEAP-Seq percent confirming: | 95.8678 |
| LEAP-Seq n confirming: | 232 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 242 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAAGGGATGCGTCGTTT |
| Suggested primer 2: | ATCTGGAGGCGCTGCTTATC |