| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066297 |
| Chromosome: | chromosome 10 |
| Location: | 1101130 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425300 | (1 of 4) 6.3.5.4 - Asparagine synthase (glutamine-hydrolyzing) / Glutamine-dependent asparagine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCCGGGCTTCAAGTCTTCACTGAAGGCAGACGGGTCGGGCGCCGCA |
| Internal bar code: | TGTTGAGACCTCACGGTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 64.7059 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGCAGAGAGTGGGACC |
| Suggested primer 2: | GCGTTGTCACCATTGTTGCT |