| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066300 |
| Chromosome: | chromosome 10 |
| Location: | 5608387 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458450 | GPX5,GPXH | Glutathione peroxidase 5; (1 of 4) 1.11.1.9 - Glutathione peroxidase | 5'UTR |
| Cre10.g458500 | ASK1,AHD2 | Aspartate kinase; (1 of 1) K00928 - aspartate kinase (lysC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGATGTTGACGCGCTGGCTATTGAGGAGTCTCTGTTATATAAACCC |
| Internal bar code: | AGTGGTAGCCGCTCTGTGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2866 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTTGAAACGCCAGCAAA |
| Suggested primer 2: | GACAGCAGGTGATGGGTTGA |