| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066305 |
| Chromosome: | chromosome 13 |
| Location: | 3753753 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCTCAATTAAACTGCGGTCGTGCTCACTCAGATTGAATGCCCCTAC |
| Internal bar code: | CAGCATCGGGGTTCAGTGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3257 |
| LEAP-Seq percent confirming: | 98.0952 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACATGCCAGCGGTGTTT |
| Suggested primer 2: | GCTCGTACCTAGAGACGCAC |