| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066309 |
| Chromosome: | chromosome 8 |
| Location: | 3497005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378900 | ND3,NUO3 | (1 of 1) K03880 - NADH-ubiquinone oxidoreductase chain 3 (ND3); NADH:ubiquinone oxidoreductase ND3 subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGCTTATTCCAACACTTGTCTGAATAGCTTATTCTCTTAGAGCGTGT |
| Internal bar code: | TCTACCACCGCGTCTAATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5255 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAATGCAACCGGGCTT |
| Suggested primer 2: | CGAGATCGCCTACCTGTTCC |