Insertion junction: CLIP2.066314_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.066314
Chromosome:chromosome 5
Location:2527530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g239300 (1 of 85) IPR009057 - Homeodomain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTCAACCCTGGCCACGAGGACTGTACTACTCCCGTTGTGCGTCAAGGC
Internal bar code:TATTGTGTTTGAGTACGGCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1251
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGATTGTCTCGCGGAA
Suggested primer 2:GCGGCTTTCGTCAAGTTTGT