| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.066327 |
| Chromosome: | chromosome 8 |
| Location: | 104684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358530 | MDE1,OPR113 | atpE mRNA stabilization factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGGAAGAGGGCGGCGTGAGCGATAGCGTGCATTGTGGTAGGTAGAAT |
| Internal bar code: | CTGGGAGATTAGCGGCGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGCTGCTCCTGCTTCT |
| Suggested primer 2: | AGGTGTGTGTGCGTATGTGT |