| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066328 |
| Chromosome: | chromosome 12 |
| Location: | 8993810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545100 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR | |
| Cre12.g545150 | (1 of 2) PTHR21514//PTHR21514:SF0 - UNCHARACTERIZED // AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGCAACGAAGCTTCCATCAGCCGACGTGGCAGGAAAGGTCTCGCAG |
| Internal bar code: | ACCGAACCTAGACTATACTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2700 |
| LEAP-Seq percent confirming: | 34.6154 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTCAGGAACTCGAATGC |
| Suggested primer 2: | ACCAACAGCAGCAGTACCAA |