Insertion junction: CLIP2.066337_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066337
Chromosome:chromosome 2
Location:6967771
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389319 (1 of 2) PTHR11266:SF17 - PROTEIN MPV17 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTAAATTTACGCCGAAGTAAACGGACACTACAACATGGCGGCTCTGA
Internal bar code:GTAGTGGTTCTCTTGGGAACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5054
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAGGAAGCAGACGTCAA
Suggested primer 2:TTCCGCGCCACAAAATTCTG